Expression of Ty-lacZfusions inSaccharomyces cerevisiae
نویسندگان
چکیده
منابع مشابه
Ty Elements of the Yeast Saccharomyces Cerevisiae
Transposable elements in the yeast Saccharomyces cerevisiae are a heterogeneous group of mobile genetic elements. Ty elements transpose via an intermediate RNA-molecule using the same replication and integration mechanisms as the metazoan retroviruses. Ty1 is a retrotransposons with life cycle very similar to that of oncogenes. This similarity provides an attractive model for virus-host interac...
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The Saccharomyces cerevisiae genome contains five families of long terminal repeat (LTR) retrotransposons, Ty1-Ty5. The sequencing of the S. cerevisiae genome provides an unprecedented opportunity to examine the patterns of molecular variation existing among the entire genomic complement of Ty retrotransposons. We report the results of an analysis of the nucleotide and amino acid sequence varia...
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We have identified mutations in seven unlinked genes (SPT genes) that affect the phenotypes of Ty and delta insertion mutations in the 5' noncoding region of the HIS4 gene of S. cerevisiae. Spt mutants were selected for suppression of his4-912 delta, a solo delta derivative of Ty912. Other Ty and delta insertions at HIS4 are suppressed by mutations in some but not all of the SPT genes. Only spt...
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Temperature-sensitive mutants of Saccharomyces cerevisiae were isolated by insertional mutagenesis using the HIS3 marked retrotransposon TyH3HIS3. In such mutants, the TyHIS3 insertions are expected to identify loci which encode genes essential for cell growth at high temperatures but dispensable at low temperatures. Five mutations were isolated and named hit for high temperature growth. The hi...
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It has been suggested that the primary evolutionary role of transposable elements is negative and parasitic. Alternatively, the target specificity and gene regulatory capabilities of many transposable elements raise the possibility that transposable element-induced mutations are more likely to be adaptively favorable than other types of mutations. Populations of Saccharomyces cerevisiae contain...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1984
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/12.3.1627